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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in lots of tumors, which ends up in chromosome instability, and therefore could lead to tumorigenesis. This protein was observed to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a important position in advertising and marketing cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are already described. Two more splice variants had been claimed but in-depth nucleotide sequence facts is just not however out there.
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Blocking peptides are peptides that bind exclusively into the target antibody and block antibody binding. These peptide usually is made up of the epitope identified from the antibody.
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This tutorial outlines additional probable optimization of cellular and immunoassay parameters to be sure the absolute best effects are...
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This tutorial outlines further more possible optimization of mobile and immunoassay parameters to ensure the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind to the epitope around the target protein. By evaluating the staining within the blocked antibody vs . the antibody alone, you can see which staining is precise.
Target Data Cyclin E belongs into the very conserved cyclin household, whose customers are characterized by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins perform as regulators of CDK kinases. Distinct cyclins exhibit unique expression and degradation designs which contribute to your temporal coordination of each mitotic celebration. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S transition. This protein accumulates within the G1-S period boundary and is also degraded as cells progress by means of S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by equally antibodies...
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